Next: Remote experiments
Up: User's Guide to Macromolecular Crystallography Experiments
Previous: Experiment policies
As per beamline policy, it is your responsibility to know and follow all safety protocols applicable to your experiment, samples and equipment you bring to the SSRL. Please note the SSRL has policies concerning the use and disposal of propane and ethane,liquid nitrogen, heavy metals and compressed gases. Please follow all the applicable rules.
To minimize the possibility of accidents while working inside the hutch, there are restrictions on the motors that can be moved from certain locations.
The hutches are equipped with oxygen deficiency sensors. An alarm will go off if the oxygen content in the hutch falls below 19.5%. This may happen while the nitrogen dewar is being refilled. Do not enter the hutch or open the door if the oxygen alarm is sounding!. If the duty operator arrives to investigate the oxygen alarm, explain that the alarm will be automatically reset once the dewar has been filled. If the alarm does not subside within a few minutes after the nitrogen filling is finished, call support staff.
The following instructions describe how to mount samples manually. For automated sample mounting, please refer to the SAM robot use instructions.
The Oxford cryostream can be used to collect data at temperatures other than 100 K. The temperature can be changed at the cryostream controller outside the hutch ( you need to ask the support staff to enable manual control of the cryojet before being able to do this):
In the case that the overhead lamp does not provide adequate illumination, the side light source can be used to better view the sample. Use the Blu-Ice interface to turn the light on and adjust the intensity of the light until you obtain a clear view of the sample. When using automated centering of the loop, the software will temporarily turn off the side light source. The software will also turn off the light if the software has been idle for some time.
On BL12-2, make sure to insert the backlight screen to be able to see small samples using the on-axis camera.
|Technical questions: Webmaster
questions: Mike Soltis
|Last modified:Wednesday, 24-Jan-2018 14:17:00 PST.|