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Macromolecular Crystallography is a technique used to study biological molecules such as proteins, viruses and nucleic acids (RNA and DNA) to a resolution higher than ~5 Å. This high resolution helps elucidate the detailed mechanism by which these macromolecules carry out their functions in living cells and organisms. Protein molecules can crystallize under regulated conditions; the crystals are made up of multiple copies of the molecule arranged in a regular 3-dimensional lattice. The x-rays deflected ("scattered") by the atoms in equivalent positions in the crystal lattice concentrate into sharp intense spots (crystal diffraction pattern). The macromolecular structure can be determined by analysis of the intensities and positions of the diffraction spots.

The Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory operates and develops beamlines providing state of the art macromolecular crystallography facilities and support for visiting researchers. Of the beamlines currently operational BL9-2 and BL14-1 are designed for MAD experiments; the side stations BL9-1, BL7-1 and BL11-1 are also MAD capable at slightly reduced energy resolution. BL12-2, with an undulator source, is optimized for microfocus applications, but can also be used for conventional experiments (MAD, screening, etc.). Researchers from universities, industry, and government laboratories around the world can gain access to the beamline facilities by submitting a research proposal. Updates on beamline facilities and other user information are posted to the px-ssrl mailing list.

News:

  • SMB staff will be at the Cold Springs Harbor Laboratory Course "X-RAY METHODS IN STRUCTURAL BIOLOGY" October 13 - 28, 2014. For more information, click here.
  • There will be a workshop on SSRL remote access data collection at the NSLS on July 17, 2014. For more information, click here.
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Beam Line 7-1 9-1 9-2 11-1 12-2 14-1
Flux @500 mA (p/s)3.5x1011
Wavelength 1.10Å
8.9x1010
Wavelength 0.98Å
6.6x1011
Wavelength 0.98Å
4.0x1011
Wavelength 0.98Å
3.3x1012
Wavelength 0.98Å
1.3x1011
Wavelength 1.22Å
ExperimentMADMADMADMAD MAD/MicrobeamMAD
Special instrumentationVisex microscopeUV-Vis microspecUV-Vis microspec
Wavelength (Å)
Energy Range (keV)
0.97-1.76
7.0-12.7
0.77-0.98
12.6-16.2
0.85-2.06
6.0-14.4
0.83-1.17
10.6-15.0
0.73-1.85
6.69-17.0
0.95-2.06
6.0-13.0
Beam Size (µm)100-250100-20050-200100-2507, 10, 20, 50-20050-200
DetectorQ315RQ315RMAR325PILATUS 6M PAD PILATUS 6M PADMAR325
Detector Size (mm2)315x315315x315325x325431x448 431x448325x325
Xtal-Detector (mm)109-65095-650102-650182-650188-90095-500
BL phone (+1-650)926-5271926-5291926-5292926-8648926-5212926-5141
Support phone (+1-650)283-4556714-9411714-9417714-9414714-9405307-4151
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