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Structural Molecular Biology at SSRL
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SSRL
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Stanford University
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Macromolecular Crystallography is a technique used to study biological molecules such as proteins, viruses and nucleic acids (RNA and DNA) to a resolution higher than ~5Å. This high resolution helps elucidate the detailed mechanism by which these macromolecules carry out their functions in living cells and organisms. Protein molecules can crystallize under regulated conditions; the crystals are made up of multiple copies of the molecule arranged in a regular 3-dimensional lattice. The x-rays deflected ("scattered") by the atoms in equivalent positions in the crystal lattice concentrate into sharp intense spots (crystal diffraction pattern). The macromolecular structure can be determined by analysis of the intensities and positions of the diffraction spots.

The Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory operates and develops beamlines providing state of the art macromolecular crystallography facilities and support for visiting researchers. Of the beamlines currently operational BL9-2 and BL14-1 are designed for MAD experiments; the side stations BL9-1, BL7-1 and BL11-1 are also MAD capable at slightly reduced energy resolution. BL12-2, with an undulator source, is optimized for microfocus applications, but can also be used for conventional experiments (MAD, screening, etc.). Researchers from universities, industry, and government laboratories around the world can gain access to the beamline facilities by submitting a research proposal. Updates on beamline facilities and other user information are posted to the px-ssrl mailing list.

News:

  • SMB staff will be at the Cold Springs Harbor Laboratory Course "X-RAY METHODS IN STRUCTURAL BIOLOGY" October 13 - 28, 2014. For more information, click here.
Beam Line 7-1 9-1 9-2 11-1 12-2 14-1
Flux @500 mA (p/s) 3.5x1011
Wavelength 1.10Å
8.9x1010
Wavelength 0.98Å
6.6x1011
Wavelength 0.98Å
4.0x1011
Wavelength 0.98Å
3.3x1012
Wavelength 0.98Å
1.3x1011
Wavelength 1.22Å
Experiment MAD MAD MAD MAD MAD/Microbeam MAD
Special instrumentation Visex microscope UV-Vis microspec UV-Vis microspec
Wavelength (Å)
Energy Range (keV)
0.97-1.76
7.0-12.7
0.77-0.98
12.6-16.2
0.85-2.06
6.0-14.4
0.83-1.17
10.6-15.0
0.73-1.85
6.69-17.0
0.95-2.06
6.0-13.0
Beam Size (µm) 100-250 100-200 50-200 100-250 7, 10, 20, 50-200 50-200
Detector Q315R Q315R MAR325 PILATUS 6M PAD PILATUS 6M PAD MAR325
Detector Size (mm2) 315x315 315x315 325x325 431x448 431x448 325x325
Xtal-Detector (mm) 109-650 95-650 102-650 182-650 188-900 95-500
BL phone (+1-650) 926-5271 926-5291 926-5292 926-8648 926-5212 926-5141
Support phone (+1-650) 283-4556 714-9411 714-9417 714-9414 714-9405 307-4151
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Participating Research Institutions
Caltech Genentech Scripps Research Institute Stanford University

Funding
Stanford University