Q. I have typed the name of the image file in the File input box, but the JPEG file is not displayed and the right frame displays ''Failed to load image header''
Make sure that the image exists (ie, no typos) and is readable.
You must type the full path to the image input window - alternatively, try using the file browser in the bottom frame.
HTTP errors may be caused by transient glitches communicating with the server. Try again. If the problem persists, tell the user support staff.
Q. In the Autoindex tab, the ''Runs'' table does not get loaded properly (the fields for the runs are empty); when trying to display the run contents, the browser displays an impersonation server error
If this problem happens, try reducing the number of runs displayed per page in the autoindex tab options in the Preferences tab. If the problem persists, there may be a large number of users trying to connect to the impersonation server. Let the user support person know about this.
Q. When I try to collect images or export a run to Blu-Ice, I get an error message saying that there are too many runs already. What does this mean?
There is a maximum limit of 16 data collection runs in Blu-Ice. When all the runs have been used up, Web-Ice will give this error message. If this happens, open a Blu-Ice window (via the NX client if you are a remote user) and delete some runs manually (doing this will NOT delete or affect the data collected by these runs).
Q. When I change the oscillation angle before Exporting the run to Blu-Ice, the exposure time changes when I recalculate the dose
The exposure time should be proportional to the oscillation angle; some people forget to adjust the former when modifying the latter, that's why the software will change it. However, if you really do intend to change the exposure per degree, just edit the exposure time. After that, you will be able to change the oscillation angle without automated corrections of the exposure time.
After autoindexing the images, LABELIT runs MOSFLM to integrate the images. This serves to confirm that the lattice chosen by LABELIT is correct and provides additional information about the crystal diffraction properties and qualities. Unfortunately, the integration step sometimes fails, particularly for poor crystals. If this happens, you can see the autoindexing results from LABELIT by following the [Details] link in the Autoindex Tab or in the Cassette Details page in the Screening Tab, and clicking on the labelit.out file.
For experienced MOSFLM users: The Details page also contains the input and output MOSFLM files (called index## and index##.out, with the number corresponding to different LABELIT solutions. Often the error will happen when integrating the triclinic solution, i.e., examine the index01.out file. You might be able to integrate the images by tweaking the input parameters in the input files.
In most cases, a system error indicates that a autoindex solution could not be found; a ''No_Indexing_Solution'' error message is given in this case, followed by the specific cause:
Occasionally Autoindex may fail with a MOSFLM error. See previous Question.
Q. My crystal is pseudo-cubic, with the true symmetry being rhombohedral. I want to use Web-Ice to calculate the phi-rotation strategy, so I tried entering the known Laue group and unit cell in to the Autoindex run setup. This failed. Autoindexing also failed when I gave just the Laue group. How can I get the right strategy?
In many cases entering the known symmetry and cell is a good way to handle pseudo-symmetry. However, this is a very special case. As shown by the LABELIT indexing log file, your crystal can be indexed in four possible rhombohedral settings, depending on which body diagonal of the primitive cell is taken as the threefold. These four cases cannot be distinguished until the data are integrated and symmetry-related intensities are compared. Still, if radiation damage is a problem, there is a way to identify the threefold with minimal initial data. Follow these steps:
Q. My buffer contains 1M NaCl and I have very small crystals. How do I account for that in the dose calculation?
See the RADDOSE documentation
If the resolution limit is lower than the target resolution, use a longer exposure time for the test images. Recollect the test images (using the Recollect button) and increase the exposure time.
|Technical questions: Webmaster
questions: Ana Gonzalez
|Last modified:Tuesday, 13-Aug-2019 14:45:10 PDT.|