Remote Access Crystallography at SSRL

Learn how to efficiently screen numerous crystals for diffraction quality and conduct successful MAD experiments without leaving your home institution.

August 4th 2006
Location: The Hauptman-Woodward Medical Research Institute, Buffalo, NY
Organizers: Aina Cohen (SSRL), Clyde Smith (SSRL), Edward Snell (HWI), Graeme Card (SSRL), Mike Hollenbeck (SSRL)

Workshop Photos

Training Summary

Complete protein crystallography experiments are carried out routinely at SSRL from remote locations around the world, using secure protocols. All the macromolecular beamlines are equipped with advanced internet-based software tools, robotic sample mounters, video observation and other highly automated equipment. In this workshop attendees will learn about the beamline and software developments and how to successfully complete all stages of a remote access experiment. The workshop is intended to be highly practical with topics covered including:

  • sample preparation for use with the Stanford automated mounting system,
  • setting up a home computer to access the Blu-Ice beam line control system,
  • sample transport,
  • automated sample screening and Web-Ice image analysis,
  • energy selection for MAD or optimized SAD experiments,
  • data collection strategy, setup, and monitoring,
  • remote data processing
  • data backup.

Schedule:  The workshop will start with lectures and a live demonstration of remote access data collection. In the afternoon there will be two hands-on training sessions:

  1. attendees will use the SSRL sample loading and shipping tools to transfer flash-cooled protein crystals into SSRL sample storage cassettes compatible with the Stanford automated sample mounter,
  2. using their own laptop computer, attendees will install the NX client software, connect to the Blu-ice beam line control system, screen crystals, plan then collect diffraction data. 


A complete users guide to data collection at SSRL with specifics to remote data collection is available at http://smb.slac.stanford.edu/public/users_guide/manual/. Publications describing some of the developments, the automated software and equipment used are available at http://smb.slac.stanford.edu/public/research/staff_publications/.

Registration:  Attendance is open to all researchers interested in conducting remote access experiments at SSRL.  Click here to access the online registration form. Space is limited in the afternoon hands-on sessions so please register as soon as possible. There will be a $70 registration fee which will include breakfast, lunch and breaks during the workshop.    For more information please send an email to ssrl_automation@hwi.buffalo.edu or a fax to the attention of Dr. Edward Snell at (716) 898-8600.

Registration Fee: The $70 registration fee should paid by July 26th.  Please download the payment form (available as either a MSWord file or as an Adobe .PDF file) and enclose this form with your payment.  Please make your check payable to the 'Hauptman-Woodward Institute' and mail it to the following address:

Remote Access Workshop/Finance Department
Hauptman-Woodward Medical Research Institute
700 Ellicott Street
Buffalo, NY 14203-1102

Directions: The workshop will be held at the Hauptman-Woodward Medical Research Institute located at 700 Ellicott Street, Buffalo, NY (map).

Accommodations:  A limited number of rooms at $89 per night for the nights of the 3rd through 5th has been arranged at the Double Tree Inn (http://www.doubletreebuffalo.com/) located within easy walking distance to HWI. (map) Please mention the Remote Access Workshop to get this rate which will be available until July 7th. Buffalo is within a short distance of Niagara Falls for those that my wish to take an opportunity for a visit before or after the workshop. http://www.niagara-usa.com/.
 

The SSRL Macromolecular Crystallography Group is supported by the DOE Offices of Basic Energy Sciences and Biological and Environmental Research, the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program, and the National Institute of General Medical Sciences.