Macromolecular Crystallography Rapid Access Application

Rapid Access Application Form

[Beam Time and Submission Dates] [Flow Chart]

Approximately five to eight shifts are reserved for Rapid Access beam time each month. Applications are reviewed by SSRL's Proposal Review Panel for scientific merit and feasibility.

The Rapid Access application may be used to request beam time for data collection during a single scheduling period.

Only one project per Principal Investigator may be scheduled for Rapid Access time within a given month. If multiple spokespersons in a group submit applications, the PI will be asked to set priority for scheduling the experiments.

Spokespersons should expect to hear back from SSRL within one week of the deadline for submittal. If the application has been approved with the highest rating among the submissions for a given deadline, beam time will be assigned within one month of the original submittal. If the application has been approved for beam time but assigned a lower rating, it will be put on a waiting list for beam time during the next block of Rapid Access time or for consideration to fill cancellations of regularly scheduled beam time.

Applications must receive a rating that equals or exceeds the average rating of the regularly scheduled proposals on BL9-2 to be awarded Rapid Access time on that beamline.

Use Agreement(s) must be fully executed between Stanford University and the spokesperson's home institution and all collaborator institutions (if personnel physically coming to SSRL) before beam time.

Spokesperson:	Institution:	Email:	Degree:

	Work Phone:	Fax:

Principal Investigator:	Email:	Work Phone:


Collaborators:	Institution: (if different from above)	Email:	Degree:


Title:


Experiment(s): multi-wavelength MAD (BLs 9-2, 1-5) single wavelength SAD (BLs 9-2, 1-5) single wavelength (tunable) (BLs 9-2, 1-5, 9-1, 11-1) single wavelength (fixed) (BLs 9-2, 1-5, 9-1, 11-1, 7-1)

Scientific Importance: (8-10 lines) State clearly what you hope to achieve and why this project is important. Will this lead to determination of a new structure, is the proposal to collect data from inhibitor complex of an already known structure, etc.? Why will these results be important?


Are crystals currently ready for data collection? Yes No

Feasibility: (8-10 lines) Projects will only be considered for Rapid Access time if well-characterized crystals are currently ready for immediate data collection. The extent of characterization will depend upon specific details of the project, but would usually include data collection on a rotating anode source. State clearly what crystals are currently ready for data collection, what data you expect to obtain, why you expect the data collection to be successful, and how the data will be used (MAD structure determination, molecular replacement, high resolution refinement, inhibitor complex, etc)


Previous Rapid Access: (8-10 lines) Fill out this section if Rapid Access time was previously awarded to the same PI during the last 12 months. Only describe the most recent Rapid Access data collection if more than one was allocated during this period. Briefly state what data were collected and their quality. What were the scientific goals and were they met -- explain how the experiment was successful or why the effort failed.

Crystal Data:

Sample Size
(mm x mm x mm) Mol. Wt.
(kD) Space Group Cell
Dimensions
(Å x Å x Å) Max
Res.
(Å) Temp. of
Data
Collection NCS* Wavelength Anom. Scattering Element Anom. Sites/
Molecule

*NCS: Number of molecules expected per asymmetric unit

AIDS Related? Yes No

Other Disease? Yes No
If yes, please specify:

Private Sector Research? Yes No
Note: Private Sector Research is subject to specific terms and conditions, and SSRL must be reimbursed at full cost recovery.

Source of Research Funds:
(hold Ctrl to select multiple options) Research Area:
(hold Ctrl to select multiple options)

SMB Sample Preparation Lab Equipment Required:

Ultracentrifuge EPR Spectrophotometer Wetlab Bench Space

Glove Box Glove Bag Fume Hood Cold Room

Auxillary Crystallographic Equipment Required:

Variable Temp. Cryostat Xe/Kr (flash freeze) Xe/Kr Cell (RT)

MSC Cryo-Xe/Kr-Siter Kappa Geometry Cold Rm. at BL
(1-5 & 11-1 only) Adjustable Incubator at BL

Other Equipment:

Safety Concerns:

YES NO	Are you bringing heavy atom solutions or any other hazardous substances to SSRL? (A hazardous material includes anything ignitable, corrosive, toxic-including carcinogens, reactive-including gases, and radioactive) If yes, list all substances and quantities:

YES NO	Are you bringing biohazards to SSRL? (infectious/pathogenic agents; viruses, toxin producing agents, r-DNA, etc.) If yes, list biohazards, quantities and NIH classifications: (ONLY CLASS 1 & 2 BIOHAZARDS MAY BE BROUGHT TO SSRL)

YES NO	Are you bringing hazardous equipment to SSRL (e.g. lasers, high voltage, high pressure, cryogenic apparatus, etc.)? If yes, please list:

	If answered yes to any of the above, please complete a hazard form.
YES NO	Have you made provisions to secure your equipment against 0.75G lateral acceleration while at SSRL?

YES NO	Will human subjects or laboratory animals be used in this experiment?
	If yes, please contact SSRL Safety Officer, Matt Padilla, 650-926-3861

SSRL User Administrative contact: L. Dunn, tel. 650-926-2087